From DNA extraction to CRISPR - master the molecular biology techniques that drive modern life sciences.

Not just theory - Learn how molecular biology experiments actually work!

Step by step, at your own pace, with expert mentor support - Get Certificate!

ADMISSIONS OPEN

The Molecular Biology Tools & Techniques – Self-Learning Training Program is a comprehensive, structured course designed to build strong conceptual understanding and practical awareness of essential molecular biology techniques used in research labs, diagnostics, genomics, and biotechnology industries.

This program bridges the gap between theory and real laboratory workflows, enabling learners to understand why, how, and when specific techniques are used—while learning at their own pace, supported by expert mentors.

About the Program

Program Type: Self-Learning (Online)
Mode: Recorded Lectures + Experiment Walkthroughs
Course Access Duration: 40 Days
Mentor Support: (Doubt Solving, guidance, feedback)
Eligibility: Life Science / Biotechnology / Microbiology / Biochemistry background (students & professionals)
Certification: Yes
Access: Online learning portal

The Molecular Biology Tools & Techniques Self Learning Program offers a structured, step-by-step learning journey covering essential experimental techniques, analytical methods, and modern molecular biology tools.

You learn through:

Curated self-learning modules
Recorded experiment walkthroughs
Hands-on data interpretation
Assignments & real experimental logic

Dedicated mentor support for doubt-clearing and guidance

Who Should Register?

B.Sc / M.Sc students in Life Sciences, Biotechnology, Microbiology, Biochemistry
Fresh graduates preparing for research or industry roles
PhD aspirants strengthening lab fundamentals
Working professionals revising molecular biology techniques
Learners planning careers in genomics, bioinformatics, diagnostics, or R&D

Detailed Curriculum

1. Fundamentals of Molecular Biology: Techniques and Analytical Calculations

Importance of molecular biology techniques
Basic and advanced molecular biology techniques
Analytical calculations:

Concentrations
Dilutions
Basic units
Unit conversions

2. Experiment Walkthrough: Calculations & Experimental Design

Explanation of calculations with worked examples
Designing experiments:

DNA extraction
Protein isolation

Serial dilution principles and calculations

3. DNA Extraction and Characterization via Agarose Gel Electrophoresis

DNA isolation from bacteria
Agarose gel electrophoresis:

Working principle
Gel staining
Band analysis and interpretation

4. Experiment Walkthrough: Agarose Gel Electrophoresis

Preparation of agarose gel
Buffer preparation
Loading dye preparation
Electrophoresis setup
Tips to minimize handling and loading errors

5. Protein Isolation & Analysis using SDS-PAGE

Protein isolation from:

Bacteria
Yeast
Plant
Animal (any one source)

SDS-PAGE:

Working principle
Gel staining
Protein band analysis

6. Experiment Walkthrough: SDS-PAGE

Preparation of reagents and buffers
Stacking gel and resolving gel preparation
Loading dye preparation
SDS-PAGE setup
Troubleshooting and error minimization

7. Introduction to Mass Spectrometry in Biomolecular Analysis

Basic principles of mass spectrometry
Components of a mass spectrometer
Workflow and steps involved
Types of mass spectrometry
Protein identification and characterization using MS

8. Quantitative & Qualitative Analysis of DNA and Proteins using UV–Visible Spectroscopy

DNA concentration and purity analysis
Protein concentration and purity analysis
UV–Visible spectroscopy:
- Principle
- Instrumentation
- Working
- Applications

9. Experiment Walkthrough: UV–Visible Spectroscopy

Preparation of DNA and protein samples
Buffer preparation
UV–Vis instrument settings
Absorbance (OD) analysis and interpretation

10. Polymerase Chain Reaction (PCR): Principles and Applications

Principle of PCR
Types of PCR
Working mechanism of basic PCR

11. Experiment Walkthrough: PCR

Preparation of DNA template, Taq polymerase, and buffers
PCR temperature and cycle settings
Analysis of PCR products using gel electrophoresis
Key experimental considerations
Amplicon number calculation

12. Primer Design for PCR

Primer design basics
Key primer design parameters
Primer design tools
Manual melting temperature (Tm) calculation

13. Sequence Alignment: Types, Methods & Tools

Basics of sequence alignment
Types of alignment
Local vs global alignment
Alignment algorithms and computational methods
Sequence alignment tools:
- Overview
- Comparison
Applications of sequence alignment
Hands-on sequence alignment and result interpretation

14. Molecular Markers: Classification and Applications

Hybridization-based markers (RFLP)
PCR-based markers:
- RAPD
- AFLP
- SNP
- VNTR
Applications of molecular markers

15. Hybridization Techniques: Principles and Applications

Southern blotting:

Principle
Process
Applications

Northern blotting:

Principle
Process
Applications

Western blotting:

Principle
Process
Applications

16. Experiment Walkthrough: Blotting Techniques

Preparation of blotting gels
Setup for Southern, Northern, and Western blotting
Buffer preparation
Antibody preparation for Western blot
Analysis and interpretation of blots

17. DNA Microarray Technology: Gene Expression & Genotyping

Principle of DNA microarray
Types of microarrays
Gene expression analysis using microarrays
Applications

18. Experiment Walkthrough: DNA Microarray

Sample preparation
Components of a DNA microarray
Reading and interpreting microarray results

19. DNA Sequencing Technologies: From Sanger to NGS

Basic steps in DNA sequencing
Sanger sequencing (automated)
Next-generation sequencing platforms:
- Illumina sequencing by synthesis
- Ion Torrent sequencing
- Nanopore sequencing
- PacBio (third-generation sequencing)

20. NGS Data Processing Pipeline

Raw data quality control
FASTQ format and Phred scores
Read alignment
Variant calling and annotation
Error correction
Challenges in long-read sequencing

21. Principles and Applications of Molecular Cloning

Major steps involved in molecular cloning
Applications of molecular cloning

22. Experiment Walkthrough: Molecular Cloning

Cloning workflow overview
Restriction enzyme digestion
Gel electrophoresis
Ligation reaction
Bacterial transformation
Colony screening

23. Bioinformatics Tools for Cloning

Plasmid mapping using SnapGene
Restriction site analysis using NEBcutter
In-silico cloning using Benchling

24. Gene Transfer Techniques: Concepts and Applications

Physical methods
Chemical methods
Biological methods

25. Experiment Walkthrough: Gene Transfer Techniques

Bacterial transformation
Eukaryotic transfection
Electroporation
Microinjection
Gene gun (biolistics)
Chemicals, buffers, voltages, and experimental parameters
Comparison of bacterial-ready vs eukaryotic-ready cells

26. CRISPR–Cas9: Mechanism and Genome Editing Applications

Origin of CRISPR-Cas9
Structure and components
Genome editing mechanism
DNA repair mechanisms:
- NHEJ
- HDR
Applications
Limitations and strategies to overcome them

Mentor Support – What Makes This Program Different

You’re not learning alone.

✔ Access to experienced mentors
✔ Concept clarification & technical guidance
✔ Assignment feedback
✔ Experimental logic explanation
✔ Career and next-step advice

Key Outcomes

By the end of this program, you will be able to:

✔ Understand and explain core molecular biology techniques
✔ Interpret experimental data confidently
✔ Connect wet-lab experiments with downstream applications
✔ Prepare for research projects, internships, and lab roles
✔ Build a strong foundation for advanced genomics & bioinformatics training

Certification

Receive a Certificate of Completion recognizing your training in Molecular Biology Tools & Techniques.

Why This Program Matters?

Modern life science careers demand more than textbook knowledge. This program is designed to help you think like a molecular biologist, understanding experimental design, data interpretation, and decision-making behind every technique.

Whether you are preparing for higher studies, research roles, or biotech and diagnostics careers, this training equips you with the conceptual depth and practical awareness expected in real-world laboratories.

Ready to Get Started?

Admissions Open

Take the next step toward strengthening your molecular biology foundation with a self-paced, mentor-supported learning experience.

Enroll now and start learning today.

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